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Reaction conditions were as followed: 50 C for 15 min (for cDNA synthesis), 95 C for 2 minutes, followed by 40 cycles of 95 C for 15 seconds, 60 C for 30 seconds

Michael Mann's Stylistic Touches Michael Mann's films are known for their heavily stylized use of color and lighting, with an especially strong affinity for the color blue

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She is an avid sports fan and Game of Thrones junkie