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To determine the levels of mRNA expression, the first-strand cDNA was amplified using KAPA SYBR FAST Universal (KAPA Biosystems) on a BioRad CFX384 Real-Time System, BioRad, California, USA

For many small businesses, this represents a significant loss of revenue

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The album was the result of a joint agreement between the German label, Ruf Records, and Stony Plain, the independent Edmonton-based label that has released his three jazz CDs

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But this current construction project, Parsley said, "is the dumbest thing the city has ever done." Complaints from both residents and drivers about traffic on Bullitt Avenue from Sixth to Ninth streets have been "numerous," said Mark Jamison, the city's traffic engineer