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After the 48 h, the supernatant was removed and a 25 mM of culture medium containing 0.5 mg/mL of MTT salt solution was added to each well and it was again adjusted in a 5% carbon dioxide incubator at 37 C for 4 h

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Conclusions: Our analyses suggest that approximately one in six respondents who reported only using e-cigarettes may have smoked tobacco cigarettes

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This tripartite validation approach was implemented to ensure methodological rigor