Primary antibodies were added, and the cells were incubated for 2 h at 37 C after washing and blocking for 30 min
This is why the recycling processsupported by magnesiumis so critical
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Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set the counter to zero with distilled Preheat Reagent II in water bath for 30 minutes: 37 (mammal cell) or 25 (other species)
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