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The biofilms in the wells were incubated at 37 C for 1 h, scraped thoroughly, and then 150 L solution from each well was transferred to a solid black microplate (PerkinElmer, USA) for top reading using a Varioskan TM LUX microplate reader (ThermoFisher, USA

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Dr Khan, former chief executive of childrens charity Barnardos, also called for a tobacco licence for retailers to limit the availability of tobacco across the country, a rethink of the way cigarette sticks and packets look to reduce their appeal, and a mass media campaign to encourage smokers to quit

The risk of developing MS in smokers may be proportional to the number of cigarettes smoked

I drive the car in an' out amongst the trees, gettin' nearer to this house, an' when I am close to the wall that goes around it I pull up

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