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Moreover, patients older than 40 years old consuming alcohol daily, or having diabetes mellitus, varicocele, chronic disease, recent infection and genetic abnormalities were excluded from the study

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Follow-up measurements As long as participants did not use any nicotine, collection of body weight, blood samples (fasting plasma glucose, blood HbA1c, serum insulin, plasma lipid profile, and plasma hsCRP), AUDIT and the SFFFQ were repeated at the end of weeks 4 and 12