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In accordance with these results, upregulation of filaggrin was also observed in oral keratinocytes of smokers 19 , in keratinocyte cell cultures 19, 20 or in reconstructed human epidermis 21 exposed to cigarette smoke extract, and porcine skin explants exposed to cigarette smoke (our unpublished data)
In a 7-month follow-up survey, 44.4% of participants said they had remained tobacco-free for at least 30 days well above the 30% success rate considered a national benchmark
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He recalls starting to regularly attend to patients at age 15, but he continued to develop his knowledge and skill in the field of healing and tobacco-based treatments throughout his life, also including periods of study with Amazonian healers other than his grandparents
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