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As it was previously mentioned, one of the main limitations of the present study is the small number of participants, which may have inuenced the internal consistency results lower than what was expected, based on the literature

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Figure 2 E-Cigarette (2.4% Nicotine) Modulates the PARP1/SIRT1 Axis PARP-1 activation and consequent NAD+ decrease are a well-established signaling pathway that mediates hepatic cell death and metabolic changes associated with DNA damage ( In prior studies using e-cigarettes, we observed hepatic steatosis (Figures 1, 2, we also found that e-cigarette (0%)-treated mice were similar to saline-treated mice