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Western blot Cells were lysed with lysis buffer (RIPA buffer from Thermo Scientific, 89900, 1X Halt protease inhibitor cocktail from Thermo Scientific, 78430, and 1X Halt phosphatase inhibitor cocktail from Thermo Scientific, 78426)

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[138] Garland would call Kennedy weekly, often ending her phone calls by singing the first few lines of "Over the Rainbow." [138] [139] [140] On August 28, 1963, Garland and other celebrities such as James Garner, Josephine Baker, Sidney Poitier, Lena Horne, Paul Newman, Rita Moreno, and Sammy Davis Jr

Limitations Limitations of the study herein include the fact that we did not use human subjects

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