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A different image is obtained by using absorptive stains such as methylene blue or Lugols solution which are directly taken up by the cells, thus staining them

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One mL of 10% trichloroacetic acid (TCA) and two mL of 0.67% thiobarbituric acid (TBA) were also added to the 0.2 mL of suspended PRBCs, which were then heated for 20 min at 90100 before being cooled

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