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We then transferred 6-L aliquots to 30 L of buffer that either favored (0.1 or 0.4 M sodium acetate pH 5.5) or prevented (0.4 M Tris-HCl pH 8.5) in vitro assembly and incubated the sample at 20 C for 56 days

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As it the seedlings drop into the ground, at least one farmers follows along and makes sure it is sewn properly into the ground