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Reads were aligned to GRCh38 and processed using GATK Best Practices for WGS, including duplicate marking, base recalibration, and variant calling via Mutect2 with matched normal-exposed pairs

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1 Nicotine also affects the development of brain circuits that control attention and learning

The survey further revealed that smoking households diverted significant resources to tobaccoaround 10.7% of the monthly budget, higher than household staples (10.4%), fruits and vegetables (6.7%), and meat (6.5%)