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The culture medium was dialysed overnight against wash buffer (50 mM sodium phosphate buffer (pH 8.0) and 20 mM imidazole)

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First launched in South Africa in 1954 and then internationally in 1957, it is sold in about 30 countries around the world

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These factors vary considerably both within and between countries, which has led to substantial inequalities in tobacco and nicotine use among youth and adults