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The inoculum was prepared from lyophilized tissue homogenized in phosphate-buffered saline (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 2 mM KH 2 PO 4 , with pH adjusted to 7.4 with HCl) at 1:5 w/v (1 g of leaf to 5 ml of PBS)

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It takes about 97 minutes for half of it to leave the body

In the name of love of a singular artist who has fronted one of the best rock bands for more than 40 years, here are 60 facts about Bono: "I'm a scribbling, cigar-smoking, wine-drinking, Bible-reading band man," he succinctly, humbly, and perhaps a little facetiously, summed himself up to Assayas