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Method of metabolomics detection and analysis For lung tissue homogenate samples, 400 l MeOH (containing 100 ng/mL Warfarin as Internal Standard) was added to the samples (100 l), which were then vortexed for 60 s, followed by centrifugation at 14,000 g and 4 for 15 min

Current electronic cigarette use among adults aged 18 and over: United States, 2021

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Spontaneous rupture of large arteries was not observed at all and, in particular, neither in the 6 patients with the COL1A1 p.(Arg312Cys) missense variant nor in 2 individuals with different COL5A1 glycine substitutions at the C-terminal domain (Additional Table 1), which represent variants previously associated with a possible higher risk of vascular events [16]