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It affects regulated BBB transport and receptor systems that are essential for normal BBB function, as well as decrease the functional activity of ion transporters (Mazzone et al., 2010)
Glutathione represents the most abundant intracellular antioxidant capable of (i) directly interacting with free radical species, (ii) revitalizing other small molecule antioxidants (e.g., vitamins C or E), (iii) maintaining the redox state of protein thiols, and (iv) serving as a cofactor for enzymes of the cellular antioxidant system (Espinosa-Diez et al
ALDH activity was determined by monitoring the formation of NADH at 340 nm during the oxidation of propionaldehyde as described 55
Unknowns remain large because human trials on the combined blend do not exist
All cell lines were maintained at 37 C and 5%CO 2
