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Bst DNA Polymerase cannot be used for thermal cycle sequencing
saving days of work in a typical cloning and expression experiment (Figure 2)
ligation occurs only at cos sites as determined by gel electrophoresis
oligo-dT priming results in a more pronounced 3´ bias than random priming
5-mM solution of ATP and a 50-mM solution of MnCl2 are included
E. cloni-10G ELITE Electrocompetent Cells (DUOs) size:12 rxns Bst DNA Polymerase cannot beE. cloni 10G and 10GF' Electrocompetent Cells Direct replacements for standard cloning strains (e. g., DH5, DH10B, JM109, TOP10, XL1 Blue, etc.) Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue white screening capable. Available in a range of high transformation efficiencies (5 109 to 4 1010 cfu g). Convenient packaging options. Lucigens E. cloni competent cells share the most useful genetic elements