Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) Avoid T1 and T5 phage

Avoid T1 and T5 phage contamination with tonA mutation

subcloning

Quality Control: MasterAmp Tth DNA Polymerase is tested for the absence of detectable nonspecific exo- and endonuclease and RNase activities

10G   ≥ 1×109 cfu/µg

Cloning of unstable DNA sequences or those expressing toxic proteins

Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) Avoid T1 and T5 phageDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease