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and wheat (e
coli to maintain CopyControl vectors at single copy until induction occurs immediately before DNA purification
Unit Definition: One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C
Note: Complete substitution of one 2´-dNTP (or one 2´-modified dNTP) for a canonical rNTP in a T7 R&DNA Polymerase reaction results in a slight decrease in yield
transcription mixture treated with 1 MBU of RNase-Free DNase I for 15 minutes at 37°C
EDTA, Iron (III), sodium salt Molecular Biology and wheat (e
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