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ApoA1 protein expressed from ClearColi BL21(DE3) competent cells was Ni-column purified and tested for endotoxicity using both the HEK-Blue cell line from Invivogen (see Fig 5) and the LAL assay (see Fig
Transformation efficiency of >1 x 1010 cfu/µg of pUC19
Comparison of endotoxin units as measured by the LAL assay using nickel-column purified recombinant ApoA1 and HSP protein expressed in ClearColi BL21(DE3) (red bars) and E
Two microliters of each of the cell lysates were separated by electrophoresis on a 1% agarose gel and the nucleic acids detected by ethidium bromide staining
Bacteria lysed with Ready-Lyse™ Lysozyme Solution
Trypsin 1:250 TransforMax EPI300 ApoA1 protein expressed from ClearColi