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5 kb gene (plasmid DNA)
Input RNA was 1 µg of Jurkat total RNA (Ambion®)
Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
Transformation efficiency comparison of E
The reagent contains disruptive and protective properties of guanidine isothiocyanate and β-mercaptoethanol to inactivate the ribonucleases present in cell extracts
Xylene DGF 5 kb gene (plasmid DNA)
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