TE Buffer (Tris-EDTA), 100X, pH 7.4 size:500mL Transformation of Endura Electrocompetent Cells

Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium (Part No 80026-1)

Plate is robust

RFXA-01/50

The ability of this mutant enzyme to incorporate certain 2´-modified-dNTPs (including but not limited to dNTPs having a 2´-fluorine or 2´-amino group) enables efficient in vitro synthesis of transcripts composed of mixed rNMP/2´-dNMP or rNMP/2´- modified-dNMP from any DNA template that is downstream of the T7 RNA polymerase promoter

Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes

TE Buffer (Tris-EDTA), 100X, pH 7.4 size:500mL Transformation of Endura Electrocompetent Cells