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M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function
2nd strand qPCR was performed using Bio-Rad iQ SYBR mastermix and gene-specific primers that yielded 250-350 bp amplicons
OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme
cloni® 5-alpha Chemically Competent Cells
was deleted from the TransforMAX EC100 strain and replaced with a modified pcnB gene that is linked to an inducible promoter
Tris HCl Buffer 1M Solution, Sterile pH 8.5 ENZYMES M-MuLV Reverse Transcriptase is an
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