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As the extent of their methylation was much lower in the brain and in lower passage astrocytes, the authors concluded that their high methylation may just be an in vitro artifact of the prolonged passaging of cells (Meissner et al., 2008), a phenomenon that was previously observed (Shen et al., 2006

This means 0.125 mL (12.5 units on an insulin syringe) delivers 0.25 mg (250 mcg)