Protein Electrophoresis size:For 6 groups of 4-5 or 24-30 students. For an RT-PCR reaction

For an RT-PCR reaction

such as cheek cells (BE-303)

Avoid T1 and T5 phage contamination with tonA mutation

This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins

analytical sample applications

Protein Electrophoresis size:For 6 groups of 4-5 or 24-30 students. For an RT-PCR reactionAn important and essential tool in protein analysis is electrophoresis. Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and or net charge on polyacrylamide gels. The proteins can be subsequently visualized with various protein stains or further analyzed by transferring to a solid membrane (Western blotted; see Western Blot Analysis kit (BE 503)). Students will learn the principles of various types