Exonuclease VII, 250 U @ 10 U/uL ISOBUC Avoid T1 and T5 phage

Avoid T1 and T5 phage contamination with tonA mutation

0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA)

No detectable DNA contamination as determined by PCR using generic primers

Colonies were inoculated into LB-Miller medium and grown at 37°C for induction

Specific: Digests ssDNA in the 5' to 3 direction

Exonuclease VII, 250 U @ 10 U/uL ISOBUC Avoid T1 and T5 phageDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease