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1X cresol red loading dye
The enzyme has no activity on nicked or closed-circular dsDNA or supercoiled DNA
Requires only 50 pg to 75 ng input of sheared/fragmented DNA
Table 1 and Figure 2 summarize transformation effectiveness
Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
Metribuzin CROSS-LINKING AND MODIFICATION 1X cresol red loading dye
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