FOCUSâ„¢ Extraction Buffer V PA * The cells contain an

* The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl capability using the EZ-Tn5™<oriV/KAN-2> Insertion Kit

A 1-hour Terminator™ Exonuclease reaction digests the large rRNAs in a eukaryotic or prokaryotic total RNA sample

AmpliTaq® (Applied Biosystems) DNA polymerase in genotyping

5´ pN—OH 3´ + Pi

The most distinctive feature of enzyme-based catalysis is its specificity and requires lock-key matching specificity before the reaction can proceed

FOCUSâ„¢ Extraction Buffer V PA * The cells contain an