Electron spin resonance spectroscopy For the analysis of in vitro samples, reactants, including the spin trap, were mixed in PBS containing diethylenetriaminepentaacetic acid (DTPA, 50 M) to a final volume of 50 l, using low protein binding tubes (90410, Thermo Fisher Scientific)
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Lyophilization is a freeze-drying process that removes water from the peptide
Iodoacetic Acid (IAA) Neurotoxicity Assays HT-22 cells were seeded into 96-well flat-bottomed plates at a density of 3000 cells/well in 100 l DMEM (high glucose, 1 mM pyruvate, 10% FBS) and allowed to attach overnight
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