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CL insufficiency marked by inadequate progesterone production, is a common cause of early pregnancy loss in both humans and domestic animals ( Epigenetic dysregulation has become one of the most important factors in the functioning of the corpus luteum (CL), especially in the regulation of steroidogenic gene activity, angiogenesis, and luteal cell survival
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Deficiency is widespread, especially in northern climates
Briefly, cells were incubated in a serum-free Dulbeccos modified Eagles medium (DMEM) containing 10 M DCFH-DA at 37 C for 30 min