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A reduced GSH status can further compromise the defense against oxidative stress and increase the oxidative modification of proteins or enzymes, causing major changes in the secondary structure that result in the impaired metabolic stability and function of modified proteins or enzymes [78,97]

Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions

Richie, thanks for once again for sharing your research with us

Surrogate improvement does not guarantee clinical benefit

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