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Briefly, the muscle biopsy was first weighed and then cut in ice-cold isolation medium (100 mM sucrose, 100 mM KCl, 50 mM Tris-HCl, 1 mM KH 2 PO 4 , 100 M EGTA, 0.1% BSA

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Co-crystal structures of GSTO1 bound to inhibitors showed a covalent association with the active site cysteine (C32) with additional hydrophilic and hydrophobic interactions in the H-site

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Mice were housed in the animal facilities at the University of Illinois at Chicago, Research Resources Center, and the studies were approved by the Institutional Animal Care and Use Committee, which is certified by the American Association of Accreditation of Laboratory Animal Care

On the other hand, GSTO1 also catalysed rapid glutathionylation of cellular proteins during oxidative stress 11,15