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Experimental studies involving diabetic ulcer models have suggested accelerated wound closure and improved tissue regeneration following GHK-Cu administration, supporting continued investigation into connective tissue repair and extracellular matrix restoration

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Cells were lysed in lysis buffer containing 10 mM Tris, pH 7.5, 100 M MgCl 2 , 5 mM -mercaptoethanol (-ME), 10 M GDP, 0.2 g/ml leupeptin, and 150 g/ml benzamidine

Then, 25 L of the mixture is placed on bottom coverslip and coved with a top coverslip