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If the supernatent contains particulates, re-centrifuge the supernatant for 15 minutes and collect the clarified second supernatant

Anti-inflamm-ageing and/or anti-age-related disease emerging treatments: a historical alchemy or revolutionary effective procedures

Ravishankar, B., Liu, H., Shinde, R., Chaudhary, K., Xiao, W., Bradley, J., Koritzinsky, M., Madaio, M

However, at lower pH and concurrent oxidative stress, membrane integrity and metabolic activity can be severely compromised as shown for other microbial species 16,48

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Klivenyi P, Calingasan NY, Starkov A, Stavrovskaya IG, Kristal BS, Yang L, Wieringa B, Beal MF (2004) Neuroprotective mechanisms of creatine occur in the absence of mitochondrial creatine kinase