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Hasan, A., Qazi, J

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For Met overexpression, cells were transfected with pCAGGS-MET plasmid using FuGENE HD (Promega) 48 h before observation

In vitro experiments To plate cells for infection, aliquots of BMDMs were thawed on ice, diluted into 9 ml of DMEM, centrifuged in an Eppendorf 5810 R at 1,200 RPM (290 x G) for 5 min, and the pellet was resuspended in 10 ml BMDM media without antibiotics

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Indeed, we observed a lower ratio of NADH to NAD + (Supplementary Fig