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Have a severe kidney or liver condition (unless cleared by their specialist physician)

Protein concentration was measured using BCA kit (Pierce) according to manufacturers instructions

As an alternative methyl donor, betaine plays a central role in maintaining methylation capacity, and its depletion may contribute to elevations in S-adenosylhomocysteine and impaired methyltransferase activity

Increase during the depressive episode and decrease with successful treatment

This window accounts for tissue depot retention beyond serum clearance (which occurs within 30 hours based on the peptides 46 hour half-life) and individual metabolic variation

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