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Superoxide measurement Cells were grown in a 12 well plate to about 50% confluence after which the media were removed and the cells were washed with HBSS (Hanks Balanced Salt Solution) followed by a 1 h pretreatment with 25 M dihydroethidium (DHE), from Life Technologies

J Diabetes Res 2016 , 2902351, (2016)

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A deficiency can cause various health issues, including megaloblastic anemia and neurological changes

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