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Although residues C32 and C88 of PfGrx are redox-inactive during the monothiol mechanism, they both facilitate the deglutathionylation of GSSR substrates

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Hanson et al., 2004), formation of the disulfide upon oxidation of roGFP2 favors the protonated, neutral form of the chromophore causing an increase in fluorescence at 395 nm while the 488 nm peak is decreased (red curve)

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