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Specifically, Its activation of either the PDE-cAMP-AMPK-SIRT1 axis and the PI3K-Akt signaling pathway highlights novel therapeutic strategies for addressing IR and glucose metabolism disturbances in PCOS

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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader