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The immunoblots were visualized using ECL Western blotting substrate (Pierce Biotechnology) on a Chemidoc XRS imager (Bio Rad) or using Dylight 800-conjugated secondary antibodies (Thermo Fisher Scientific, Rockford, IL) on an Odyssey Imaging Systems (LI-COR Biosciences, Lincoln, NE)
Interaction with the insulin receptor and other growth factors like Mechano Growth Factor (MGF) IGF-1 LR3 also interacts with the insulin receptor, which plays a critical role in regulating blood glucose levels and promoting cell growth throughout the body
Less tyrosinase, less pigmentation
Siegel D, Devaraj S, Mitra A, et al
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation