Interestingly, opiate withdrawal appeared more resistant to intervention, requiring multiple DSIP administrations over an extended period for observable effects
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Rather than flooding synapses with a single neurotransmitter, it modulates the balance between serotonin, dopamine, and norepinephrine, nudging each toward optimal levels rather than artificially elevating one at the expense of others
Cells were collected and washed with PBS once, and cellular fluorescence was determined by flow cytometry and data were collected by BD FACSCanto and analyzed with FlowJo V.10.1 software
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