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Measurement of liver tissue level of malondialdehyde (MDA), glutathione, and superoxide dismutase) After preparing the tissue homogenate, the double-sandwich enzyme-linked immunoassay (ELISA) technique is used to measure MDA, GSH, and SOD concentration in the supernatant for each marker from MyBioSource, USA, using an automated ELISA reader (ELISA-humareder , Germany)