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In all cases, the ligand: receptor mixtures were incubated for 30 min at 15 C and the HTRF signal recorded using a Cary Eclipse spectrofluorimeter (Varian) with an excitation wavelength set at 337 nm and an emission wavelength at 665 and 620 nm

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After that, they were fixed in a 10% formaldehyde buffered solution

Mahajan T, Crown A, Checkley S, Farmer A, Lightman S

Frenette M, Scaiano JC