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The cell suspension was washed and centrifuged twice at 1,000 rpm at 4C and resuspended in DMEM enriched with 2 mM glutamine, 50 g/l gentamicin sulfate, 10% fetal bovine serum, and 1 mM mercaptoethanol

Epidemiological evidence in some cases hints that the accepted dosages of vitamin B helps only to avoid their marginal deficiency and further benefits could accrue from higher dosages than those provided by RDA (4-5)

Maximal effects typically occur at four to six weeks, with full tissue remodeling continuing over three to six months

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Ehrlich HP, et al

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